Quantitative Analysis of a-Mangostin in Fruit Hulls of Garcinia Mangostana Extract and it’s Content in Formulation by a Validated RP-HPLC Method

The present study involves the optimization of different solvent extract and Standardization of α-mangostin from fruit hulls of Garcinia mangostana was studied by gradient reverse-phase high-performance liquid chromatographic (HPLC) method for quality control and quantity determination. Chromatographic separation was carried out on Develosil RP C-18 (5 μm, 4.6 x 150mm) at room temperature using a (Gradient phase) mobile phase consisting of flow rate of 1.0mL/min, with UV detection at 245nm. The method has been studied for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The linearity of the proposed method was found in the range of 10-200 ug mL with regression coefficient 0.9998. The method was also applied for the determination of mangostin in three samples collected from three different places and two herbal formulation. The proposed HPLC method was simple, accurate, precise and it is suitable for performing the quality control of crude extract and herbal formulation.